An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

June 2013 (No. 2)

Effects of post-thaw incubation on motility, acrosomal integrity and in vitro fertilizing capacity of boar spermatozoa cryopreserved in 0.5 and 5 ml straws

Alejandro Córdova a,b, Rocío Hernández-Gil c, Cristian Alejandro Córdova-Jiménez d, Diogo José Cardilli d, Kellen de Sousa Oliveira d, José Félix Pérez-Gutiérrez d,*

a Department of Agricultural and Animal Production, Metropolitan Autonomous University, Mexico;

b Academic Division of Agricultural Sciences, Juarez Autonomous University of Tabasco, Mexico;

c Kubus S.A., Las Rozas de Madrid, Madrid, Spain;

d Department of Animal Medicine and Surgery, Complutense University of Madrid, Madrid, Spain

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Abstract
The effect of post-thaw incubation (0 vs. 5 h at 15 8C) and straw size (5 vs. 0.5 ml) on motility, acrosomal integrity and in vitro fertilizing (IVF) capacity of cryopreserved boar spermatozoa was studied. In samples assessed immediately after thawing, no differences were found between the two straw sizes. After 5 h post-thaw incubation, all parameters, except polyspermy, decreased and, spermatozoa packaged in 5 ml straws showed better functional and IVF parameters than these in 0.5 ml straws.

Reproductive Biology 2013 13 2: 166–168.

* Corresponding author: Departamento de Medicina y Cirugía Animal, Hospital Clínico Veterinario, Facultad de Veterinaria, Universidad Complutense de Madrid, Av. Puerta de Hierro s/n, 28040 Madrid, Spain; e-mail address: jfperez@vet.ucm.es