An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

September 2013 (No. 3)

Evaluation of Tris–citric acid, skim milk and sodium citrate extenders for liquid storage of Punjab Urial (Ovis vignei punjabiensis) spermatozoa

Bushra A. Rakha a,* Iftikhar Hussain a, Shamim Akhter b, Nemat Ullah c, Syed M.H. Andrabi b, Muhammad S. Ansari d

a Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, Pakistan

b Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, Pakistan

c Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46000, Pakistan

d Department of Zoology, University of Gujrat, Gujrat 50700, Pakistan

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Abstract
The Punjab Urial (Ovis vignei punjabiensis) is an endangered subspecie of ovidae, distributed as small scattered populations in the forest belt of the Himalayan foothills of Pakistan and in the areas enclosed by the Indus and the Jhelum rivers. The present study was conducted to evaluate the liquid storage of Punjab Urial spermatozoa in different extenders for use in future in situ conservation activities. Semen was collected by electro-ejaculation from three captive Punjab Urial rams. Suitable ejaculates of individual animals were pooled and divided into three aliquots for dilution with the experimental extenders (Tris–citric acid, skim milk and sodium citrate) at 37 °C. Extended semen was cooled from 37 °C to 5 °C in 2 h, and stored for three days at 5 °C. Sperm motility (%), viability (%; live/dead), acrosome integrity (%) and plasma membrane integrity (%) were assessed on days 1, 2 and 3 of storage. On day 1, sperm motility, viability as well as acrosome and plasma membrane integrity were similar (p > 0.05) in all three experimental extenders. On day 2, sperm motility, viability, acrosome and plasma membrane integrity were higher (p < 0.05) in Tris–citric acid extender compared to sodium citrate based extender. On day 3 of storage, the values of motility, viability and acrosome integrity were higher (p < 0.05) in Tris–citric acid extender than in skim milk and sodium citrate based extenders. In conclusion, Tris–citric acid extender appears to be a better option compared with skim milk and sodium citrate extenders for liquid storage of Punjab Urial semen.

Reproductive Biology 2013 13 3: 238–242.

* Corresponding author: Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Rawalpindi 46300, Pakistan; E-mail addresses: bushrauaar@gmail.com, arbushra@uaar.edu.pk (B.A. Rakha).