An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

December 2013 (No. 4)

Sex-sorting of boar spermatozoa does not influence the localization of glucose transporters

Diego Bucci *, Giovanna Galeati, Elisa Giaretta, Carlo Tamanini, Marcella Spinaci

Dipartimento di Scienze Mediche Veterinarie (DIMEVET), Università degli Studi di Bologna, Via Tolara di Sopra, 50, 40064 Ozzano dell’Emilia, Bologna, Italy

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Abstract
Sex-sorting damages spermatozoa function, shortening their lifespan and fertility. This study used an immunofluorescence technique to investigate the effect of sex-sorting on the localization of glucose transporters (GLUTs) in boar spermatozoa. GLUTs are trans-mem- brane proteins responsible for glucose transport within cells. Distribution of GLUTs on sperm cells was similar in unsorted and sex-sorted semen, suggesting that the flow cytometric sex-sorting process did not affect the sperm energy apparatus.

Reproductive Biology 2013 13 4: 341–343.

* Corresponding author: diego.bucci3@unibo.it, diego.bucci1@gmail.com (D. Bucci).