An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

September 2013 (No. 3)

The utility of Brilliant Cresyl Blue (BCB) staining of mammalian oocytes used for in vitro embryo production (IVP)

Jolanta Opiela a,* Lucyna Kątska-Książkiewicz b

a National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, Balice/Kraków, Poland

b Department of Animal Physiology and Breeding, Branch Campus of the Faculty of Biotechnology, Rzeszów University, Kolbuszowa, Poland

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Abstract
The present article summarizes the results of experiments investigating the Brilliant Cresyl Blue (BCB) staining for selection of immature oocytes before in vitro embryo production or somatic cell nuclear transfer. Developmental competence of oocytes stained with BCB and quality of blastocysts derived from such oocytes as well as the expression of apoptosisrelated genes, mitochondrial DNA (mtDNA) replication-related genes and the transcripts encoded by the mitochondrial genome in BCB stained oocytes are discussed.

Reproductive Biology 2013 13 3: 176-183.

* Corresponding author: National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 1 Krakowska St., Balice/Kraków, Poland; E-mail address: jopiela@izoo.krakow.pl (J. Opiela).