September 2014 (No. 3)
Precision-cut luteal slices: A promising approach for studying luteal function in pigs
Emilia Przygrodzka, Marcelina Lopinska, Adam J. Ziecik*
Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research of PAS, Bydgoska 7 Str., 10-243 Olsztyn, Poland
The study was aimed to validate the precision-cut luteal slices to investigate porcine luteal function. Corpora lutea (CLs) were cut into 180-µm thick slices using Krumdick Tissue Slicer. The viability, tissue structure and steroidogenic acute regulatory protein (STAR) expression in the luteal slices did not differ between the beginning and the end of the 24-h incubation period. The luteal progesterone secretion showed a time- and dose-dependent response to porcine luteinizing hormone. The effects of prostaglandin F2α and 17β-estradiol on proges- terone secretion by porcine luteal slices were comparable to the previously reported in vivo results of the CL microdialysis system in the pig.
Reproductive Biology 2014 14 3: 243-247.
* Corresponding author: E-mail address: firstname.lastname@example.org (A.J. Ziecik)