An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

December 2014 (No. 4) Volume 14

Human placenta-derived Wnt-5a induces the expression of ICAM-1 and VCAM-1 in CD133+CD34+-hematopoietic progenitor cells

Friederike Herr a, Manuela Horndasch b, Désirée Howe b,c, Nelli Baal b,d, Pankaj Goyal a,e, Silvia Fischer f, Marek Zygmunt a,1, Klaus T. Preissner f,1,*

a Department of Obstetrics and Gynecology, Ernst-Moritz-Arndt-University, D-17475 Greifswald, Germany

b Department of Obstetrics & Gynecology, Justus-Liebig-University, D-35385 Giessen, Germany

c Department of Anesthesiology, Justus-Liebig-University, D-35385 Giessen, Germany

d Institute for Clinical Immunology & Transfusion Medicine, Justus-Liebig-University, D-35385 Giessen, Germany

e Department of Biotechnology, School of Life Sciences, Central University of Rajasthan NH-8, Bandar Sindri, Distt. Ajmer, Rajasthan 305801, India

f Institute of Biochemistry, Medical School, Justus-Liebig-University, D-35392 Giessen, Germany

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Abstract

Angiogenesis and vascular development are essential for fetal development and growth, whereby early pregnancy loss and other pregnancy-related pathologies have been linked to aberrant vascular development. As Wnt signalling has been suggested to play a role in the vascularization of chorionic villi, we investigated the expression of Wnt family members in trophoblasts and stromal cells isolated from chorionic villi of early placenta and the influence of Wnt signalling on CD133+CD34+-hematopoietic progenitor (CD133+CD34+) cells. Wnt-5a was expressed in human placental stromal cells and to a lesser extent in human trophoblast cells. rWnt-5a impeded migration and induced adhesion of CD133+CD34+ cells, in accordance with the expression of adhesion proteins, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). rWnt-5a-related regulation of the ICAM-1 and VCAM-1 expression were dependent on the release of Ca2+ and the activation of transcription factor – nuclear factor of activated T-cells (NF-AT). We propose that Wnt-5a is required during early placenta development to mediate adhesion and homing of CD133+CD34+ cells.

Reproductive Biology, 2014 14 (4): 262-275.

* Corresponding author at: Institute of Biochemistry, Medical School, Justus-Liebig-University, Friedrichstrasse 24, 35392 Giessen, Germany.
E-mail address: klaus.t.preissner@biochemie.med.uni-giessen.de (K.T. Preissner).

1 Equal senior authors