An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

December 2014 (No. 4) Volume 14

Local transfer of testosterone and aromatase activity in the spermatic cord in wild boar/pig hybrids in short-daylight and long-daylight periods

Anna Tabęcka-Łonczyńska a,c,*, Przemysław Gilun b, Stanisława Stefańczyk-Krzymowska b, Marek Koziorowski a,c

a Department of Animal Physiology and Reproduction, University of Rzeszów, ul. Rejtana 16C, 35-959 Rzeszów, Poland

b Department of Local Physiological Regulation, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

c Centre of Applied Biotechnology and Basic Sciences, University of Rzeszow, Werynia, 36-100 Kolbuszowa, Poland

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Abstract

The local transfer of testosterone (T) and immunolocalization of cytochrome P450 aromatase (P450arom) in the spermatic cord vessels of ten male wild boar × domestic pig hybrids were examined in December (short-daylight period) and June (long-daylight period). Total T concentration was determined in the jugular vein (JV) and free T concentration was estimated in the common carotid artery (CA), branches of the testicular artery supplying the testis (TA) and epididymis (EA), as well as in testicular veins draining blood from the testis (TV) and spermatic cord (SV). P450arom was immunolocalized in the arterial and venous vessels of the spermatic cord. The concentrations of total T in the JV and free T in the CA did not differ between the examined periods. However, in December, free T concentrations in the TA and EA were higher ( p < 0.01–0.001) than in the CA. In June, free T concentration was higher ( p < 0.01) in EA than in CA and TA. The concentrations of free T in the TV and SV were higher ( p < 0.001) than in the JV regardless of the period. Also, free T concentration in the SV was higher ( p < 0.05) in June than in December. P450arom was expressed in all layers of the arterial and venous vessels of the spermatic cord. In June, the intensity of the P450arom staining was higher than in December. The results suggest that the local supply of the male reproductive organs with steroid hormones operate in the hybrids of wild boar × domestic pig. This supply includes the local transfer of testosterone and the P450arom action.

Reproductive Biology, 2014 14 (4): 282-288.

* Corresponding author at: Department of Animal Physiology and Reproduction, University of Rzeszów, ul. Rejtana 16C, 35-959 Rzeszów, Poland. E-mail address: annaurz@wp.pl (A. Tabęcka-Łonczyńska)