An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

December 2014 (No. 4) Volume 14

Morphological evaluation of sperm from infertile men selected by magnetic activated cell sorting (MACS)

Gianni Curti d, Fernanda Skowronek a, Rita Vernochi c, Ana Laura Rodriguez-Buzzi c, Juan Carlos Rodriguez-Buzzi c, Gabriela Casanova b, Rossana Sapiro a,*

a Laboratorio de Biología Molecular de la Reproducción – Departamento de Histología y Embriología, Facultad de Medicina, Gral. Flores 2125, 11800 Montevideo, Uruguay

b Unidad de Microscopía Electrónica de Transmisión – Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay

c Centro de Reproducción Humana del Interior (CERHIN), Bvar. Artigas 1665/102, Montevideo, Uruguay

d Departamento de Biologia Molecular – Instituto De Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, CP 11600 Montevideo, Uruguay

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Abstract

Electron microscopy analysis performed in five infertile human subjects after sperm selec-tion by swim-up followed by magnetic activated cell sorting (MACS) demonstrated a decrease in the number of spermatozoa with characteristics compatible with cell death. However, no significant differences were found when the swim-up/MACS semen fraction was compared with swim-up fraction alone.

Reproductive Biology, 2014 14 (4): 289-292.

* Corresponding author. E-mail addresses: giannicurti@gmail.com (G. Curti), fernandaskowronek@gmail.com (F. Skowronek), draritaverno@gmail.com (R. Vernochi), anucharod@gmail.com (A.L. Rodriguez-Buzzi), jcrbuzzi@gmail.com (J.C. Rodriguez-Buzzi), paragaby@gmail.com (G. Casanova), rsapiro@fmed.edu.uy, rossanasapiro@gmail.com (R. Sapiro)