March 2015 (No. 1) Volume 15
Caffeine stimulates in vitro pituitary LH secretion in lipopolysaccharide-treated ewes
Andrzej Przemysław Herman a,*, Anna Herman b, Janina Skipor c, Agata Krawczyńska a, Joanna Bochenek a, Dorota Tomaszewska-Zaremba a
a Polish Academy of Sciences, The Kielanowski Institute of Animal Physiology and Nutrition, 05-110 Jabłonna, Poland
b The Academy of Cosmetics and Health Care, 13 Podwale Street, 00-252 Warsaw, Poland
c Polish Academy of Sciences, Institute of Animal Reproduction and Food Research, 10 Tuwima Street, 10-747 Olsztyn, Poland
The study was designed to determine the effects of caffeine on luteinizing hormone (LH) secretion and gene expression of caffeine-associated receptors in anterior pituitary (AP) explants obtained from saline- and lipopolysaccharide (LPS)-treated ewes. Animals had been treated with LPS or saline daily for seven days. Three hours after the last injection of LPS/saline, the AP were collected and divided into four explants. The explants were incubated with: 1/medium-199 (control explants), 2/gonadotropin-releasing hormone (GnRH; 100 pmol/mL; a positive control), 3/caffeine (10 mmol/L), or 4/GnRH + caffeine. Caffeine stimulated (p < 0.05) LH release by explants from both saline (19.7 vs. control 12.6 ng/mg) and LPS (28.3 vs. control 13.9 ng/mg) treated animals. The effect of caffeine on LH secretion was stronger in the LPS-treated group than in saline-treated group, and the observed LH release was similar to that induced by GnRH alone (27.2 ng/mg). Caffeine increased (p < 0.05) LHb gene expression only in explants from LPS-treated animals. In conclusion, the results of the present study demonstrated a stimulatory in vitro effect of caffeine on LH secretion by ovine pituitary explants. The potency of the caffeine-induced LH secretion was affected by in vivo treatment of the animals with endotoxin. Reproductive Biology 2015, 15, 1: 20-26 .
* Corresponding author: e-mail address: firstname.lastname@example.org (A.P. Herman)