An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

March 2015 (No. 1) Volume 15

Caffeine stimulates in vitro pituitary LH secretion in lipopolysaccharide-treated ewes

Andrzej Przemysław Herman a,*, Anna Herman b, Janina Skipor c, Agata Krawczyńska a, Joanna Bochenek a, Dorota Tomaszewska-Zaremba a

a Polish Academy of Sciences, The Kielanowski Institute of Animal Physiology and Nutrition, 05-110 Jabłonna, Poland

b The Academy of Cosmetics and Health Care, 13 Podwale Street, 00-252 Warsaw, Poland

c Polish Academy of Sciences, Institute of Animal Reproduction and Food Research, 10 Tuwima Street, 10-747 Olsztyn, Poland

Full text on

Abstract

The study was designed to determine the effects of caffeine on luteinizing hormone (LH) secretion and gene expression of caffeine-associated receptors in anterior pituitary (AP) explants obtained from saline- and lipopolysaccharide (LPS)-treated ewes. Animals had been treated with LPS or saline daily for seven days. Three hours after the last injection of LPS/saline, the AP were collected and divided into four explants. The explants were incubated with: 1/medium-199 (control explants), 2/gonadotropin-releasing hormone (GnRH; 100 pmol/mL; a positive control), 3/caffeine (10 mmol/L), or 4/GnRH + caffeine. Caffeine stimulated (p < 0.05) LH release by explants from both saline (19.7 vs. control 12.6 ng/mg) and LPS (28.3 vs. control 13.9 ng/mg) treated animals. The effect of caffeine on LH secretion was stronger in the LPS-treated group than in saline-treated group, and the observed LH release was similar to that induced by GnRH alone (27.2 ng/mg). Caffeine increased (p < 0.05) LHb gene expression only in explants from LPS-treated animals. In conclusion, the results of the present study demonstrated a stimulatory in vitro effect of caffeine on LH secretion by ovine pituitary explants. The potency of the caffeine-induced LH secretion was affected by in vivo treatment of the animals with endotoxin.  Reproductive Biology 2015, 15, 1: 20-26 .

* Corresponding author: e-mail address: andrewherman@wp.pl (A.P. Herman)