An official journal of the Society for Biology of Reproduction and the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
Elsevier

June 2015 (No. 1)

Tauroursodeoxycholic acid improves the implantation and live-birth rates of mouse embryos

Tao Lin a, Yun Fei Diao a,1, Jung Won Kang a, Jae Eun Lee a, Dong Kyu Kim b, Dong Il Jin a,*

a Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Republic of Korea

b National Institute of Animal Science, Rural Development Administration, Suwon, Republic of Korea

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Abstract

We previously demonstrated that tauroursodeoxycholic acid (TUDCA) improved the developmental competence of mouse embryos by attenuating endoplasmic reticulum (ER) stress-induced apoptosis during preimplantation development. Here, we present a follow-up study examining whether TUDCA enhances the implantation and live-birth rate of mouse embryos. Mouse 2-cell embryos were collected by oviduct flushing and cultured in the presence or absence of 50 µM TUDCA. After culture (52 h), blastocysts were transferred to 2.5-day pseudopregnant foster mothers. It was found that the rates of pregnancy and implantation as well as the number of live pups per surrogate mouse were significantly higher in the TUDCA-treated group compared to the control group, but there was no significant difference in the mean weights of the pups or placentae. Thus, we report for the first time that TUDCA supplementation of the embryo culture medium increased the implantation and livebirth rates of transferred mouse embryos.

Reproductive Biology 2015, 15 (2):

* Corresponding author at: Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon, Republic of Korea.  E-mail address: dijin@cnu.ac.kr (D.I. Jin).

1 Present address: Institute of Special Animal & Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun,   30112, China